Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1981.
The isolation and characterization of Escherichia coli mutator genes have led to a better understanding of DNA replication fidelity mechanisms and to the discovery of important DNA repair pathways and their relationship to spontaneous mutagenesis. Mutator strains in a population of cells can be beneficial in that they allow rapid selection of variants during periods Cited by: Escherichia coli and other species of Escherichia ferment various sugars producing acid with gas.. But these sugar fermentation tests are of no diagnostic value in routine laboratory tests except sucrose fermentation test, However, that is also not well understood and some strains are able to ferment sucrose as well, but it can be used in differentiating Escherichia coli. Mutation Research Elsevier Publishing Company, Amsterdam Printed in The Netherlands 35 CHEMICAL MUTAGENESIS IN E. COLI B/R; THE INFLUENCE OF REPAIR SYSTEMS FOR UV DAMAGE C. H. CLARKE School of Biological Sciences, University of East Anglia, Norwich (Great Britain) (Received January I6th, ) SUMMARY Broth-grown cells of the Escherichia coli Cited by: Direct Selection for Mutators in Escherichia coli JEFFREY H. MILLER,* ANJALI SUTHAR, JENNIFER TAI, ANNIE YEUNG, CINDY TRUONG, AND JEAN LEE STEWART Department of Microbiology and Molecular Genetics and The Molecular Biology Institute, University of California, Los Angeles, California Received 28 August /Accepted 8 December
We demonstrate that this system can be used to evolve antibiotic resistance in wild-type E. coli in chemical mutagens, ultraviolet light and the mutator . Abstract. An azaserine-resistant derivative of Escherichia coli B/UV, AZA/R 1, was found to carry a mutator gene, designated mutS1, was mapped by means of conjugation and P1kc-mediated mutS1 gene was cotransduced with argB at a frequency of %; the gene order in this region of the chromosome is thy argB determine whether a . Our lake has been tested for E. Coli and the results were given as such: 1: > 2: 5. 3: 5. and 4: I assume they did 5 separate tests but did not explain if these are high or low and whether our is contaminated to the point that we cannot swim in or uuse its water to take showers or let the dogs in it since they drink the water. Table of Contents hide Biochemical Test of Escherichia coli (E. coli) Fermentation of Enzymatic Reactions Biochemical Test of Escherichia coli (E. coli) Share the article on:Biochemical Test of Escherichia coli (E. coli) Basic Characteristics Properties (E. coli) Capsule Capsulated Catalase Positive (+ve) Citrate Negative (-ve) Coagulase Negative (-ve) .
Escherichia coli detection for food and beverage safety: Rapid testing solutions and regulatory guidance to avoid contamination. According to the U.S. Center for Disease Control (CDC), the large and diverse bacterial group of gram-positive, rod shaped Escherichia coli, (E. coli) contain numerous mammal-associated strains with many harmless, some beneficial, and some . The argE3 → Arg + reversion system in Escherichia coli K Escherichia coli K12 was isolated from the stool of a convalescent diphtheria patient in USA (Palo Alto, California) in and deposited in the strain collection of the Department of Bacteriology of Stanford University. Serological studies revealed that after many years of cultivation under . In this system, a plasmid encoding full-length MvfR was mutagenized in the Escherichia coli XL1-Red mutator strain (Agilent Technologies, Inc.) (35), and the . Abstract. The mutagenic and toxic effects of a series of N-alkyl-N'-nitro-N-nitrosoguanidines were examined in Escherichia coli K The role of nucleotide excision repair, the SOS response, and the adaptive response in both the reduction and the production of the biological effects of these chemicals was tested.